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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has long been observed in many tumors, which results in chromosome instability, and thus may well add to tumorigenesis. This protein was located to associate with, and be involved with, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in cell-cycle regulated histone gene expression and performs a crucial part in promoting mobile-cycle development in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, are already described. Two more splice variants were being described but in-depth nucleotide sequence details is just not nevertheless available.
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Blocking peptides are peptides that bind specifically on the target antibody and block antibody binding. These peptide normally has the epitope acknowledged by the antibody.
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This tutorial outlines additional possible optimization of mobile and immunoassay parameters to be certain the very best final results are...
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This tutorial outlines further more doable optimization of mobile and immunoassay parameters to guarantee the absolute best final results are...
Antibodies certain to the blocking peptide no more bind on the epitope within the concentrate on protein. By evaluating the staining in the blocked antibody vs . the antibody by yourself, one can see which staining is certain.
Goal Information and facts Cyclin E belongs for the very conserved cyclin household, whose customers are characterized by a extraordinary periodicity in protein abundance through the mobile cycle. Cyclins functionality as regulators of CDK kinases. Distinctive cyclins exhibit distinct expression and degradation designs which lead to your temporal coordination of each and every mitotic celebration. Cyclin E sorts a complex with and capabilities as being a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S changeover. This protein accumulates for the G1-S stage boundary and is degraded as cells development via S stage.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by each antibodies...
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